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ISA 2006 authentication problem

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Dear,

I am setting up ISA 2006 as a web proxy server.
I am using the Single Network Adapter template and I am using Active Directory Integrated authentication.  when i using domain username login to a computer,  they can access the internet. but there are about one or two times (about 5 minitues ) interupt  everyday. how can i fix this problem? Thanks very much.

and it pop up a window let me to input username and password, but i input the correct username and password, it still pop up a window. while i cancle the window ,it show the follow issue:

407 Proxy Authentication Required。The ISA Server requires authorization to fulfill the request. Access to the Web Proxy filter is denied. (12209)


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